How do I dilute xGen Custom Hyb Panels from IDT for the Targeted Gene Expression Assay?

Question: How do I dilute xGen Custom Hyb Panels from IDT for the Targeted Gene Expression Assay?

Answer: IDT’s xGen Custom Hyb Panels are compatible as fully custom panels or as add-ons to the 10x pre-designed panels in the Targeted Gene Expression assay. There are three formats of xGen Custom Hyb Panels:

• xGen Custom Hyb Panel - Accel
• Faster turnaround time and lower cost
• xGen Custom Hyb Panel
• Includes xGen NGS functional test report 
• xGen Custom Hyb Panel - Production
• Includes xGen NGS functional test report and quality documentation

Selecting the appropriate IDT panel format for the application is important for optimal results and minimizing cost. Please refer to IDT’s website for more information regarding the different panel formats.

The xGen Custom Hyb Panels are new offerings from IDT. We are updating our User Guides and other documentation to reflect these new offerings. In the meantime, please refer to the guidance below on how to dilute the panels for use as add-on or fully custom panels in the Targeted Gene Expression Assay.

For Add-on Genes to Pre-designed Panels

Follow the instructions in step 2.1A of either the Targeted Gene Expression - Single Cell User Guide or the Targeted Gene Expression - Spatial User Guide. Table 2b below replaces Table 2 and includes the working dilutions for any of the 3 formats of the IDT xGen Custom Hyb Panels.

Table 2b: Generation of add-on panel working dilutions (20amol/bait/ul)

*Volume from Table 1 in the User Guide

For Fully Custom Panels (Single Cell only)

Follow the instructions in step 2.1A of the Targeted Gene Expression - Single Cell User Guide.

Table 1b replaces Table 1 in the Fully Custom Panel section of step 2.1A. 

Table 1b: Dilution of IDT xGen Custom Hyb Panels

*panels containing up to ~60,000 baits have been tested by 10x Genomics.

Products: Targeted Gene Expression