Question: Can manually generated cDNA from the Chromium Controller or Chromium X Series be brought onto the Connect for Automated Library Construction?
Answer: For supported protocols, manually generated cDNA can be brought onto the Chromium Connect for the Automated Library Construction workflow. Examine the appropriate assay user guide for cDNA preparation and cycling guidelines.
Our current supported kits for library construction from manual cDNA on the Connect are included in the list below:
- Chromium Next GEM Single Cell 3' LT Kit v3.1
- Chromium Next GEM Single Cell 3' GEM, Library & Gel Bead Kit v3.1
- Chromium Next GEM Single Cell 5ʹ Kit v2
For guidance on how to use cDNA generating from the GEM-X assays, please see the following article: Can automated libraries be made on the Chromium Connect from GEM-X cDNA?
Products: Chromium Connect