Question: How much cDNA needs to be carried over onto the system for Automated Library Construction?
Answer: For 3’ generated cDNA, the system will require carrying over 15 uL of cDNA into column 7 of the full-skirted plate. For 5’ generated cDNA, the system requires 5-22 uL targeted at a mass of 60 ng to be carried over and placed in column 7 of the full-skirted plate.
Products: Chromium Connect