Question: What consumables are supported for use with the Chromium Connect Instrument?
Answer: Validated consumables are outlined in the Single Cell Gene Expression and Single Cell Immune Profiling User Guides.
- Chromium Next GEM Automated Single Cell 3ʹ Reagent Kits User Guide (v3.1 Chemistry)
- Chromium Next GEM Automated Single Cell 5ʹ Reagent Kits User Guide (v2 Chemistry)
Alternative compatible part numbers are listed below. Please note that using unsupported plates and tips not outlined in the User Guide or this article risks decreased assay performance and instrument failure. Therefore, consumable substitution is not supported or recommended.
Plates
- Eppendorf 951020401 twin.tec® 150 µl PCR Plate, 96-well, full-skirted - colorless
- Eppendorf 951020427 twin.tec® 150 µl PCR Plate, 96-well, full-skirted - yellow
- Eppendorf 951020443 twin.tec® 150 µl PCR Plate, 96-well, full-skirted - green
- Eppendorf 951020401 twin.tec® 150 µl PCR Plate, 96-well, full-skirted - blue
- Eppendorf 951020486 twin.tec® 150 µl PCR Plate, 96-well, full-skirted - red
- Labforce Amplifyt™ 96-Well PCR Plates, Full-Skirted, Clear
- PN #1149K05, also known as PN 485096 (available from Thomas Scientific)
- or Catalog No. NC1959287, NC1959288 from Fisher Scientific (25/case or 100/case)
** please note: when using the Amplifyt full-skirted plate on your Connect instrument, please make sure the plate is fully-seated in its block on the instrument deck by pressing down when placing the plate on-deck
- Eppendorf 951020303 twin.tec® 250 µl PCR Plate, 96-well, semi-skirted- colorless
- Eppendorf 951020320 twin.tec® 250 µl PCR Plate, 96-well, semi-skirted- yellow
- Eppendorf 951020346 twin.tec® 250 µl PCR Plate, 96-well, semi-skirted- green
- Eppendorf 951020362 twin.tec® 250 µl PCR Plate, 96-well, semi-skirted- blue
- Eppendorf 951020389 twin.tec® 250 µl PCR Plate, 96-well, semi-skirted- red
Tips
- No validated tip alternatives are available at this time for the Hamilton tips outlined in the User Guides.
We are aware that there is a shortage of both Hamilton tips and Eppendorf plates due to supply chain issues at this time. We recommend setting up consumable ordering before experimental planning. 10x is in the process of validating additional alternatives and will update this page when these are available.
Products: Chromium Connect