Question: Can antibody labeling be performed after CMO labeling when using the 3' CellPlex kit for Cell Multiplexing?
Answer: This is theoretically possible.
In our Demonstrated Protocol for CMO Labeling (CG000391), we provide protocols for labeling cells with both antibodies and CMOs. The cells are first labeled with antibodies targeting Cell Surface Proteins and then labeled with CMOs.
It may theoretically be possible to change the labeling order, to first label with CMOs, then pool the CMO-labeled samples together, and then label with antibodies. However, we have not validated this in-house and therefore cannot guarantee assay performance. Please reach out to Support if you are attempting this.
When considering any modifications to our Demonstrated Protocol, please note that adequate washing after labeling is essential to remove background from unbound CMOs and unbound antibodies.
Products: Single Cell Gene Expression, CellPlex