Question: Are there differences in microfluidic failure rates (clogs and wetting failures) between the standard and HT chips?
Answer: Microfluidic failures (clogs and wetting failures) are most commonly the result of sample quality. Samples that have a high degree of debris, cell aggregates, dead cells, or cells larger than 50 microns are likely to experience a higher risk of microfluidic failures.
- HT chips are carefully optimized for high throughput sample processing with a multiplet rate approximately half that of the standard assay, allowing for recovery of 2X the number of cells in the same amount of time as a standard chip. As a byproduct of the increased throughput on the HT chip, the failure rate for a given sample on the standard chip will increase approximately 2-fold when loading 2x as many cells on an HT chip.
Products: Single Cell Gene Expression, Single Cell Immune Profiling