Question: My thermal cycler lid temperature doesn't go lower than 50°C, what should I do?
Answer: We understand that the lid temperature of some of the validated thermal cyclers for Visium and Xenium don't go lower than 50°C but some steps of the Visium and Xenium FFPE protocols calls for lower lid temperatures. For those steps that require lower lid temperatures, we would advise turning off the lid heating but keeping the lid closed during incubation.
Products: Visium for FFPE, Xenium In Situ Gene Expression