Question: Why are there so many drying steps in the Visium for FFPE v1 Demonstrated Protocols?
Answer: We have included drying steps within our Demonstrated Protocols to maximize tissue adhesion to the Visium Slide. These steps are included: after tissue placement, during Deparaffinization, and after Coverslip Removal. We recognize that these additional drying steps lead to a longer protocol and can be inconvenient, but we would not advise excluding or shortening these steps.
It is important to note that over-drying can lead to cracking or blackening of tissue. As long as the tissue is immediately coverslipped after staining, this should mitigate tissue artifacts due to over-drying.
Products: Visium for FFPE