Question: Should I punch and re-embed my FFPE block so my tissue can fit within the Capture Area?
Answer: We would not advise punching and re-embedding your FFPE block so that the tissue can fit within the Capture Area of the Visium or Xenium Slide. We found that generating punches and re-embedding lead to a decrease in assay performance. Therefore, we would instead recommend scoring the block.
Visium for FFPE
Details on scoring blocks to 6.5 mm x 6.5 mm to fit within the Capture Area can be found in the Visium Spatial Gene Expression for FFPE – Tissue Preparation Guide.
Xenium
Details on scoring blocks to 10.45 mm x 22.45 mm to fit within the available sample positioning area can be found in the Xenium In Situ for FFPE – Tissue Preparation Guide.
Products: Visium for FFPE, Xenium In Situ Gene Expression