Question: Should I punch and re-embed my FFPE block so my tissue can fit within the Capture Area?
Answer: We would not advise punching and re-embedding your FFPE block so that the tissue can fit within the Capture Area of the Visium Slide. We found that generating punches and re-embedding lead to a decrease in assay performance. Therefore, we would instead recommend scoring the block so that fits within the 6.5 mm x 6.5 mm Capture Area. Details on scoring blocks are in the Visium for FFPE Tissue Preparation Guide.
Products: Visium for FFPE