Question: When do I need to purchase a Library Construction Kit for HT?
Answer: Additional library construction kits may be required when running the Chromium Next GEM Single Cell 5’ HT v2 kit. Each Chromium Next GEM Single Cell 5' Kit v2 contains enough library preparation materials to make either one or two libraries (either gene expression, TCR or BCR) per gel bead reaction, depending on the kit size (48 rxn or 8 rxn, respectively). If you wish to make additional libraries per gel bead reaction, an additional Library Construction Kit will be required. For more information on library construction kit considerations and further guidance, please see 'When do I need an additional Library Construction Kit to run my experiment with the Next GEM 5’ HT v2 reagent kits?'.
For the Chromium Next GEM Single Cell 3' HT v3.1 kit, each kit contains enough library preparation material to make one library prep gel bead reaction (gene expression). If feature barcode or targeted gene expression assays are added, you would need to purchase additional kits such as the 3ʹ Feature Barcode Library Kit (PN-1000262). How many Feature Barcode kits do I need for HT? For targeted gene expression, we have information on the assay here.
Products: Single Cell Immune profiling, Single Cell gene Expression, Targeted Gene Expression