Question: What should I do if I have an odd number of samples?
Answer: To enable high throughput sample processing, the HT chips were significantly redesigned. Each gel bead reaction now feeds into two sample wells. If an odd number of sample wells are run, this will result in the loss of one gel bead reaction.
In the event an odd number of samples is obtained (for example, in the unlikely event a sample is lost during sample processing), we recommend one of the following:
- Running another sample across two different sample wells (preferred).
- Running a mock sample by preparing the master mix and adding water (86.4 ul) in place of the single-cell suspension. Partitioning oil should be added to the recovery well as you would do for a real sample. In this instance, the gel beads for this mock sample reaction would go to waste.
Please do not add 50% glycerol in the sample well or the recovery well, as this may compromise assay performance and risk chip failure.
Products: Single Cell Gene Expression, Single Cell Immune Profiling