Question: Can I use the Dual Index Kit Plate TT Set A, 96 rxns (PN-1000215) for Sample Index PCR with Visium for FFPE?
Answer: The Dual Index Kit Plate TTSet A, 96 rxns (PN-1000215) is not compatible with Visium for FFPE. The dual index plates contain primers that anneal to the Illumina adapters. Different index plates containing different primer sequences are needed based on which Illumina adapters are present in the library. For example:
- Dual Index Plate TT Set A - contains Illumina TruSeq Read 1 and TruSeq Read 2 sequences.
- Dual Index Plate TS Set A - contains Illumina TruSeq Read 1 and TruSeq Small RNA Read 2S sequences.
If the incorrect index plate is used, library preparation will fail. Please refer to the appropriate User Guide and use only the specified index plate for your library type.
Therefore, in order to be able to add these required sequences, you will need to purchase the Dual Index Kit Plate TS Set A, 96 rxns (PN-1000251).
Products: Visium for FFPE