Question: What does a good Visium Spatial Gene Expression for FFPE library look like?
Answer: The trace of a good Visium library will appear as a single discrete peak with an average library size of ~240 bp. You may observe very small low molecular weight peaks (50 to 150 bp size range). These are low levels of primer carryover remaining after SPRIselect cleanup. This is normal and should not affect sequencing or application performance.
Library traces are provided below. Traces are also provided in the Visium Spatial Gene Expression for FFPE User Guide.
BioAnalyzer - DNA High Sensitivity Kit
Tapestation - High Sensitivity D5000 Kit
LabChip GX Touch HT - DNA HS Reagent Kit
Products: Visium for FFPE