Question: Can Visium for FFPE libraries be pooled with other dual indexed 10x libraries?
Answer: We have tested pooling Visium for FFPE libraries with dual index 3’ v3.1 libraries and saw no impact in library quality when limiting Visium for FFPE libraries to <40% of the total sequencing pool. It would, therefore, be low risk to pool Visium for FFPE libraries with other dual index libraries that have the same sequencing configuration as dual index 3' v3.1 libraries (e.g. Visium for fresh-frozen).
If pooling with other libraries (that require the following configuration: Read 1 - 28 cycles, i5 index - 10 cycles, i7 index - 10 cycles, and Read 2 - 90 cycles), Read 2S will need to be increased to 90 cycles.
Products: Visium for FFPE