Question: Can I purchase nuclei buffer for my ATAC or Multiome experiment?
Answer: The nuclei buffer is not sold separately. There are two tubes of 20x Nuclei Buffer in the ATACv2 kit. it is included in the kit. For nuclei/sample prep optimization purposes, the nuclei buffer is not needed. PBS can be used for final nuclei resuspension.
Be sure to use the diluted nuclei buffer in the final experiment, however. Each tube of 20x Nuclei Buffer contains enough to make ~32ml of working dilution(1x) for final nuclei resuspension. Typical usage is up to 1 ml per sample.
Products: Single Cell ATAC, Single Cell Multiome ATAC + GEX