Question: Can immunofluorescence staining and imaging have a negative impact on Visium for FFPE v1 assay sensitivity?
Answer: The Visium for FFPE Immunofluorescence Staining Demonstrated Protocol will likely result in a decrease in the number of unique transcripts detected for many tissue types with no impact on the fraction of reads mapped confidently to the filtered probe set, as compared to the Visium for FFPE H&E Staining Decrosslinking Protocol. However, this should not affect the interpretation of experimental results.
Products: Visium for FFPE