Question: Does the Visium for FFPE Immunofluorescence Demonstrated Protocol differ from standard Immunofluorescence protocols?
Answer: Yes, some components of the Visium for FFPE Immunofluorescence Demonstrated Protocol deviate from standard immunofluorescence protocols such as the wash buffer used, antigen retrieval/decrosslinking incubation temperature, and antibody incubation time. Customers should perform the immunofluorescence staining as outlined in the Demonstrated Protocol. The protocol has been optimized for optimal RNA quality to perform spatial gene expression analysis.
Prior to performing IF staining on Visium Spatial Gene Expression Slides, confirm the compatibility of the decrosslinking and buffer conditions with antibodies of interest using plain glass slides by following the Demonstrated Protocol.
Product: Visium for FFPE