Question: Can I make Single index GEX libraries using the 10x Multiome ATAC + RNA assay?
Answer: Theoretically, it may be possible to generate single index GEX libraries for the Multiome assay. However, we do not support it.
Please keep in mind that to generate single index GEX libraries, you would also need the "SI Primer". This primer is not included in the Multiome kit. This primer is also available for purchase on its own without buying the parent kit. Details about the primer concentration and purification methods are proprietary information.
For Gene Expression libraries, the SI Primer and Single Index Plate T Set A are used to prepare Single Index libraries, whereas the Dual Index Plate TT Set A is used to prepare Dual Index libraries. Please refer to the image below showing the primers and index plates needed.
Products: Single Cell Multiome ATAC + GEX