Question: What are the recommended sequencing specifications for Visium for FFPE libraries?
Answer: We recommend sequencing at least 25k paired-end reads per tissue-covered spot. Visium libraries should be run in paired-end, dual index configuration. We recommend the following cycling conditions: Read 1 - 28 cycles, i5 index - 10 cycles, i7 index - 10 cycles, and Read 2 - 50 cycles.
Products: Visium for FFPE