Question: What can I do if the Fiducial Frame is dim during fluorescence imaging?
Answer: The Fiducial Frame signal may be weaker than the antibody fluorophore signal.
- If using the TRITC channel for antibody staining, capture two TRITC channels. One TRITC channel will be for antibody staining, and the second will be exposed for the fiducial frame.
- If creating multiple TRITC channels within the same acquisition is impossible, you can create an ancillary image either using TRITC or a brightfield image. For details on these approaches, please see the Visium for FFPE Imaging Guidelines Technical Note.
Products: Visium for FFPE