Question: Can I use alternative methods to qPCR such as Nanodrop or Qubit for Cycle Number Determination during the Visium for FFPE v1 Gene Expression workflow?
Answer: qPCR is the only validated method to quantify unamplified Probe Extension Products accurately and can't be replaced with alternative methods. The concentration of cDNA and Probe Extension Product is below the detection level for alternative methods; therefore, qPCR has to be employed to quantitate how many cycles should be performed for Sample Index PCR.
Products: Visium for FFPE