Question: Is my antibody compatible with the Visium for FFPE Immunofluorescence (IF) Staining Demonstrated Protocol?
Answer: Optimal decrosslinking and staining conditions for a particular tissue will be dependent on the antigen, fixation method, the primary antibody used, and other factors. We utilize broadly applicable decrosslinking conditions and similar buffer conditions to a standard IF staining. Prior to performing IF staining on Visium Spatial Gene Expression Slides, confirm the compatibility of the decrosslinking and buffer conditions with your antibodies of interest using plain glass slides by following the Immunofluorescence Staining Demonstrated Protocol.
Products: Visium for FFPE