Question: I can’t purchase the recommended TE buffer, pH 9.0 (Genemed, PN: 10-0046), are there any alternatives that could be used?
Answer: If you cannot purchase the recommended buffer, we would advise making it using Tris Base and EDTA. The recipe is in the Visium for FFPE H&E and Immunofluorescence Staining Demonstrated Protocols.
If you'd like to use an alternative commercially available TE buffer for decrosslinking, please check that the pH is 9 and that there are no additives or detergents. Additional reagents in the buffer besides Tris and EDTA are undesirable and may impact assay performance. If required, please adjust the buffer pH to 9 by following the instructions linked above. If using an alternative, please keep in mind it would be untested and we cannot guarantee assay performance.
Products: Visium for FFPE