Question: I can’t purchase the recommended TE Buffer, pH 9.0 (Genemed, PN: 10-0046), are there any alternatives that could be used?
Answer: Alternative vendors of TE Buffer (pH 9.0) investigated by our R&D team have additives that can negatively impact assay performance and therefore, we wouldn’t recommend using them.
If you cannot purchase the recommended butter, we would advise making it using Tris Base and EDTA. The recipe is in the Visium for FFPE H&E and Immunofluorescence Staining Demonstrated Protocols.
Products: Spatial Gene Expression for FFPE