Question: What sample handling methods can be implemented to minimize the likelihood of FFPE tissue detachment on Visium Slides?
Answer: Tissue adhesion to slides is impacted by tissue processing (fixation and embedding) and other factors such as sample handling. Below are some best practices related to sample handling that should minimize the risk of detachment:
- Allow the tissue block sufficient time in the ice bath to ensure proper hydration. Dehydrated blocks are prone to cracking and sections that are cracked can impact adhesion.
- Section tissue at 5 µm. Thicker sections are more prone to detachment.
- Allow the section to float in the water bath until it is free of folds and wrinkles. Sections with folds are more likely to detach.
- Ensure that there are no bubbles under the tissue section prior to Slide placement. Bubbles under sections can negatively impact adhesion.
- The sections on the slides should be uniform without any cracks or tears. Cracks and tears can impact image quality and attachment.
- Dry sections at 42°C for 3 hours and place Slides at room temperature in a slide mailer with desiccant pouches or a desiccator overnight before staining. Failure to efficiently dry sections can increase the risk of detachment.
Products: Spatial Gene Expression for FFPE