Question: What tissue section thickness should I use for the Visium for FFPE assay?
Answer: For the Visium for FFPE assay, 4-8 μm section thickness has been tested in-house. When analyzing the data generated, we found similar assay sensitivity using 4 µm as compared to 8 µm sections. High-quality sections at 4 µm thickness can be difficult to generate, while thicker sections are more prone to detachment. Therefore, we strongly recommend 5 μm sections.
Products: Spatial Gene Expression for FFPE