Question: How do I determine if my tissue block will be compatible with the Visium for FFPE assay?
Answer: We are recommending that customers perform an RNA Quality Assessment of their tissue block of interest before the Visium for FFPE Gene Expression assay. Based on in-house experiments, we've found that as the DV200 metric drops lower than 50%, the reliability of generating high-quality gene expression data also declines.
Therefore, we recommend that customers use tissue blocks with DV200 ≥50%. Details on how to perform RNA Quality Assessment are in our Tissue Preparation Guide.
Products: Spatial Gene Expression for FFPE