Question: When using different FFPE blocks, what is the best way to section and prepare the Visium Gene Expression Slide with tissue?
Answer: When using different FFPE blocks, we recommend placing all four tissue sections on a Visium Slide in a single sectioning session.
- Perform sectioning when all blocks are available and prepare the slide with individual tissue sections. We recommend changing blades between each tissue type.
- The Visium Spatial Gene Expression Slide with tissue sections placed should be dried at 42°C for 3 hours and can be stored at room temperature in a desiccator overnight.
- The tissue is stable for up to 2 weeks before proceeding with the Visium for FFPE workflow.
Products: Visium for FFPE