Question: How many Slide Seals are required for the Staining Demonstrated Protocols and Visium for FFPE v1 workflow?
Answer: The Visium for FFPE Staining Demonstrated Protocols require at least 1 Slide Seal per Visium Slide. If performing Immunofluorescence optimizations before the Gene Expression workflow, additional Slide Seals may be required. The Visium for FFPE User Guide will require 5 Slide Seals per Visium Slide.
Customers should count the number of Slide Seals they have in their Slide Kit before starting the workflow. To generate new or resize Visium Slide Seals, use one of the provided Seals (PN-2000283/2000284) as a template to cut additional seals from MicroSeal 'B' PCR Plate Sealing Film (PN-MSB1001; listed in Additional Kits, Reagents & Equipment). Contact support@10xgenomics.com if assistance is required.
Products: Visium for FFPE