Question: What are the validated stopping points during the Visium HD 3' Spatial Gene Expression assay workflow?
Answer: Below are a list of stopping points validated for the Sample Preparation & Library Construction protocols of the Visium HD 3' Spatial Gene Expression assay. Multiple stopping points have been built into the workflow to maximize flexibility. Please refer to the Visium HD 3' protocols for preparation & specifics regarding storage conditions at each of these stopping points.
- Validated Stopping Points during the Visium HD 3' Tissue Preparation Handbook (CG000804) steps:
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- After Separate Tissue Freezing & Embedding (Step 1.2), frozen tissue blocks can be stored at -80°C for long-term storage & after OCT embedding frozen tissue blocks, they can be stored at -80°C for long-term storage.
- After Simultaneous Tissue Freezing & Embedding (Step 1.3), Frozen, OCT embedded tissue blocks can be stored at -80°C for long-term storage.
- After Section Placement (Step 1.5), tissue slides can then be stored at -80°C for up to 4 weeks.
- After Coverslip Mounting (Step 2.7), tissue slides can be stored laying flat in a slide holder at 4°C for up to 24 hours.
- After Imaging (Step 2.8), tissue slides can be stored laying flat in a slide holder at 4°C for up to 72 hours.
- Validated Stopping Points during the Visium HD 3' User Guide (CG000805) steps:
- After Second Strand Synthesis (Step 4.1), tissue slides can be stored at 4°C for up to 24 hours.
- After cDNA Amplification (Step 5.1), samples can be stored at 4°C for up to 24 hours.
- After cDNA Amplification SPRIselect cleanup (Step 5.2), samples can be stored at 4°C for up to 72 hours, or -20°C for up to 4 weeks.
- After Sample Index PCR (Step 6.5), samples can be stored at 4°C for up to 72 hours.
- After Sample Index PCR SPRIselect cleanup (Step 6.6), samples can be stored at 4°C for up to 72 hours, or -20°C for long-term storage.
Product: Visium HD 3'