Question: Is it normal to observe settling of gel beads before loading the chip in Chromium assays?
Answer: Prior to loading the chip, gel beads are vortexed and briefly centrifuged. After centrifugation, some settling of gel beads may be observed. This is normal and does not impact assay performance. We do not recommend additional mixing of the gel beads if settling occurs and the chip is loaded promptly.
A representative image of gel beads after vortexing and centrifugation is shown below. Appearance may vary based on the assay and version.
Products: Universal 3' Gene Expression, Universal 5' Gene Expression, Flex Gene Expression, Epi ATAC, Epi Multiome ATAC + Gene Expression.