Question: Can I reduce the volume of CMO used for labeling when using the 3’ CellPlex kit for Cell Multiplexing?
Answer: We do not recommend decreasing the volume of CMO used for labeling. We recommend using 100 µl of CMO per labeling reaction as outlined in our Demonstrated Protocol. The concentration and volume of CMO was optimized to ensure robust labeling of cells/nuclei within our validated input range (0.1-2 million cells/nuclei). Decreasing the volume of CMO may result in inefficient labeling and may decrease the accuracy of CMO tag assignment in Cell Ranger.
Products: Single Cell Gene Expression, CellPlex