Question: Are MACS microbeads compatible with the 3’ CellPlex Kit for Cell Multiplexing?
Answer: This is theoretically possible but untested.
Microbeads are compatible with our Single Cell 3' Gene Expression assay, as described here. However, we have not yet tested microbeads in combination with our 3' CellPlex assay.
It may be possible to label cells with Cell Multiplexing Oligos (CMOs), wash to remove background from unbound CMOs, pool the CMO-labeled cells together (CG000391), and then use the pooled sample as input into Miltenyi Biotech bead-based protocols for positive or negative enrichment of cells, prior to loading the cells onto the 10x Genomics chip.
We anticipate that CMO-labeled cells would be compatible with microbead-based assays; however, we cannot guarantee assay performance since we have not specifically tested this.
Also, see this article for guidelines on the stability of CMO tags in pooled samples.
Products: Single Cell Gene Expression, CellPlex