Question: Does the number of input libraries impact assay performance in the Targeted Gene Expression assay?
Answer: The Targeted Gene Expression assay was designed for optimal performance over a range of library inputs, ranging from a single input library (300 ng) to a pool of eight input libraries (2,400 ng). During in-house testing, we observed excellent assay performance when using library inputs within this range (one to eight input libraries). We found that overall data quality and targeting metrics were comparable regardless of the number of input libraries used.
Products: Targeted Gene Expression