Question: Is cell counting different for the Next GEM Single Cell 3’ v3.1 LT assay?
Answer: The Next GEM Single Cell 3’ v3.1 LT assay allows for the same cell preparation and cell counting techniques as our standard 3’ v3.1 gene expression assay. When preparing a single cell suspension, customers should continue to use our best practices for optimal cell counting.
For accurate cell recovery using the 3’ v3.1 LT application, a single cell/nuclei suspension should be prepared as per guidelines provided in Demonstrated Protocols on the 10x Genomics Support site and diluted to the optimal loading concentration (100-600 cells/µl) prior to chip loading. Cells should be diluted using only compatible media/buffer and minimize carryover of media/buffer that can destabilize GEMs or impact GEM-RT reaction.
Products: Single Cell Gene Expression