Question: Why is the cell recovery rate different for the Next GEM Single Cell 3’ LT v3.1 assay relative to other 10x Genomics single-cell applications?
Answer: The 3' LT v3.1 kit contains reagents and consumables that are distinct from the standard Single Cell Gene Expression kit. The LT kit has been specifically developed to deliver cost-effective single-cell data for lower throughput applications, such as pilot studies and optimization of experimental protocols and conditions. As a result, the expected cell recovery rate is not equivalent to the standard kit.
Data generated from the Single Cell Gene Expression LT v3.1 product are comparable to those from the standard Single Cell Gene Expression product, and the results and insights can be used to inform the design of full scale studies.
Further details about cell recovery-specific changes to the v3.1 LT reagents and consumables are proprietary.
Products: Single Cell Gene Expression