Question: Can I recover more than 1000 cells using the Next GEM Single Cell 3’ LT v3.1 assay?
Answer: The Next GEM Single Cell 3’ LT v3.1 assay has a supported cell recovery of 100 - 1000 cells. Loading more cells than validated will result in increases in multiplet rate.
A 1:1 mixture of fresh frozen human HEK293T and mouse NIH3T3 cells was used in a classic experiment to demonstrate single-cell behavior using the Next GEM Single Cell 3’ LT v3.1 assay. At 1,701 cells recovered, the data resulted in an elevated multiplet rate of 14.5%.
Products: Single Cell Gene Expression