Question: What sample pooling ratios are supported when using the 3’ CellPlex Kit for Cell Multiplexing?
Answer: When pooling cell or nuclei samples, we recommend using equal pooling ratios (1:1) as this allows for optimal multiplet detection. However, it is technically possible to pool samples at unequal ratios. We support pooling ratios ranging from 50%:50% to 95%:5%.
Pooling ratios below 5% are not supported. If one sample represents fewer than 5% of the total cells, Cell Multiplexing tag assignment in Cell Ranger may not be accurate. This is because Cell Ranger requires sufficient data per tag to set appropriate thresholds for tag assignment.
Products: Single Cell Gene Expression