Question: Why was the part number updated for the 3’ Feature Barcode kit?
Answer: The part number for the 3’ Feature Barcode kit was updated from PN-1000276 to PN-1000262. This was associated with the release of the Cell Multiplexing assay in March 2021.
The part number for the 3’ Feature Barcode Kit was updated because new reagents were added for the preparation of Cell Multiplexing libraries:
- The kit was updated to include Feature cDNA Primers 3, which are cDNA amplification primers compatible with the Cell Multiplexing assay.
- The kit was also updated to contain one additional tube of Amp Mix, to ensure that enough Amp Mix is available to prepare Cell Multiplexing libraries in addition to Cell Surface Protein or CRISPR Screening libraries.
If you plan to generate Cell Multiplexing libraries, it is essential to purchase the 3’ Feature Barcode Kit with the new part number (PN-1000262).
If you do not plan to generate Cell Multiplexing libraries and only plan to generate Cell Surface Protein or CRISPR Screening libraries, you can continue to use the previous 3’ Feature Barcode Kit (PN-1000276) or you can use the new kit (PN-1000262).
Products: Single Cell Gene Expression, CellPlex