Question: What is the recommended number of CMO tags when using the 3’ CellPlex Kit for Cell Multiplexing?
Answer: For optimal cell multiplet detection, we recommend using 1 tag for every ~2,500 cells.
*30,000-60,000 Targeted Cell Recovery is supported for the HT assay only.
For further details on how the number of CMO tags affects cell multiplet detection rates, please see: Can cell multiplets be filtered out when using the 3’ CellPlex Kit for Cell Multiplexing?
To increase the number of tags used in a given experiment, you can split each sample into aliquots and label each aliquot separately with a different tag. See: Can I use more than one CMO tag per sample?
Products: Single Cell Gene Expression, CellPlex