Question: How many cell multiplets will remain undetected in my final data when using the 3' CellPlex Kit for Cell Multiplexing?
Answer: These tables (below) shows the expected number of detected and undetected cell multiplets at different Targeted Cell Recoveries and with different numbers of CMO tags.
Detected multiplets = multiplets that contain two or more cells with different CMO tags
Undetected multiplets = multiplets that contain two or more cells with the same CMO tag
These table assumes that samples are pooled at equal ratios. Pooling samples at unequal ratios will decrease multiplet detection.
3' v3.1 (standard) assay:
3' HT v3.1 assay:
What affects the cell multiplet rate and cell multiplet filtering?
Products: Single Cell Gene Expression, CellPlex