Question: Are methanol-fixed cells compatible with the 3' CellPlex Kit for Cell Multiplexing?
Answer: We have not tested the 3' CellPlex Kit on methanol-fixed cells and do not support it.
Given that methanol-fixed cells are fragile after methanol fixation and rehydration, the sample quality will likely be compromised if methanol-fixed cells are subjected to the additional handling steps associated with CMO labeling and washing.
Also, we have not tested whether methanol-fixation interferes with the ability of the lipid-conjugated CMOs to label the cell membrane.
Products: Single Cell Gene Expression, CellPlex