Question: How many cells do I need for CMO labeling when using the 3' CellPlex Kit for Cell Multiplexing?
Answer: The Demonstrated Protocol for Cell Multiplexing Oligo (CMO) labeling was validated for use with 100,000 to 2,000,000 input cells or nuclei. If the number of cells/nuclei is not limited, we recommend using at least 500,000 as input.
We do not recommend performing CMO labeling if you have fewer than 100,000 cells/nuclei per sample. The CMO labeling protocol includes multiple wash steps, which are essential to remove unbound CMO background. We expect to lose between 30% and 70% of the starting cells/nuclei during these wash steps. Therefore, if fewer than 100,000 cells/nuclei are used as input, there may not be sufficient cells/nuclei remaining after CMO labeling for accurate cell counting, pooling, and loading onto the chip.
Products: Single Cell Gene Expression, CellPlex