Question: Can I use an alternative to the KAPA SYBR Fast qPCR Master Mix (KK4600) for cDNA cycle number determination during the Visium Gene Expression workflow?
Answer: We DO NOT recommend using an alternative SYBR qPCR Master Mix for cDNA cycle number determination. Only the KAPA reagent has been validated with the Visium Gene Expression assay. The KAPA SYBR Mix contains a fast DNA polymerase allowing for cycle number determination within 25 cycles. We have done limited testing with alternative SYBR qPCR mixes, and they failed to yield comparable results.
In the United States, the KAPA SYBR Fast qPCR Master Mix can be ordered directly from Roche. In Europe and the Asiatic regions, this product can be ordered from Millipore Sigma. In Canada, depending upon the territory, it may be available from either Roche or Millipore Sigma.