Question: Can RIN score be used for sample quality estimation before starting the Single Multiome ATAC + GEX assay?
Answer: RIN score is not a good measure of experiment success in the Single Cell Multiome ATAC + GEX assay. This is because the RIN score which measures RNA integrity is a bulk measurement and does not discern between cytoplasmic or nuclear RNA transcripts.
Bulk RNA can be extracted from tissues and RIN score test may be performed to determine the quality of RNA in your tissue. A low RIN score could indicate RNA degradation thus making it challenging to get good quality Multiome GEX data. However, a high RIN score is not a guarantee of success in the Single Cell Multiome assay. You still have to ensure you have a clean, high-quality nuclei suspension as input to the Single Cell Multiome ATAC + GEX assay. In summary, the RIN score is inconclusive as quality control for the Single Cell Multiome ATAC + GEX assay.
Products: Single Cell Multiome ATAC + GEX