Question: Which 10x Genomics assays are compatible with the 3’ CellPlex Kit for Cell Multiplexing?
Answer: The 10x Genomics 3’ CellPlex Kit is a multiplexing solution for use with the Chromium Single Cell 3’ Gene Expression assay and the Chromium Single Cell 3' Gene Expression HT assay.
At this time, we do not have any Cell Multiplexing solutions that are supported for use with the following assays:
- Single Cell Immune Profiling
- Single Cell ATAC
- Single Cell Multiome ATAC + Gene Expression
The 3’ CellPlex kit contains oligo-conjugated lipids with a sequence complementary to “Capture Sequence 2” on the gel bead oligos in the 3' v3.1 and 3' HT v3.1 kits. This allows the oligo-conjugated lipids to be captured by 3’ v3.1 or 3' HT v3.1 gel beads. The gel beads in other products (Single Cell Immune Profiling, ATAC, and Multiome) do not contain “Capture Sequence 2”. Therefore, they will not be able to capture the oligo-conjugated lipids in the 3’ CellPlex Kit.
We are currently developing a 5’ CellPlex Kit compatible with the Single Cell Immune Profiling assay. However, we do not yet have a timeline for when this kit will be released.
Products: Single Cell Gene Expression, Single Cell Immune profiling, CellPlex