Question: Can I pool more than 12 samples together when using the 3’ CellPlex Kit for Cell Multiplexing?
Answer: No, we do not support pooling more than 12 cell or nuclei samples together when using the 3’ CellPlex kit.
The 3’ CellPlex Kit contains 12 Cell Multiplexing Oligos (CMO's), each having a unique sample barcode. This kit can be used to label and pool up to 12 cell or nuclei samples.
While it may be technically feasible to design additional CMOs to label and pool more than 12 samples, this is not supported by 10x Genomics. We do not offer any protocols for designing and synthesizing additional CMO's. The type of lipid included in the CMO's is proprietary.
When analyzing data in Cell Ranger, the Cell Multiplexing pipelines do not support the analysis of more than 12 pooled samples.
Products: Single Cell Gene Expression, CellPlex