Q: When do I need an additional Single Cell 5' Library Construction Kit (PN-1000020) to run my experiment with Single Cell VDJ v1 or v1.1 reagent kits?
A: Each Chromium Single Cell VDJ v1 or v1.1 kit contains enough library preparation materials to make one library per gel bead reaction (either gene expression, TCR or BCR). If you wish to make additional libraries per gel bead reaction (for example, if you wish to make both gene expression and TCR libraries), an additional Library Construction Kit will be required.
The number of gel bead reactions and the number of libraries you want to make per gel bead reaction will determine the number of additional Single Cell 5' Library Construction Kits needed. Each Single Cell 5' Library Construction Kit (PN-1000020) contains enough material to make 16 libraries. Please see the table below for examples of when additional library construction kits are needed.
|
Library types run |
Number of Gel Bead Reactions purchased |
Is an additional Single Cell 5' Library Construction Kit (PN-1000020) required? |
Number of additional 5' lib construction rxns required |
Number of additional 5' lib construction kits required* |
|
GEX only/ TCR only/ BCR only |
4 |
No |
0 |
0 |
|
GEX + TCR/ GEX + BCR/ TCR + BCR |
4 |
Yes |
4 |
0.25 |
|
GEX + TCR + BCR |
4 |
Yes |
8 |
0.5 |
|
GEX only/ TCR only/ BCR only |
16 |
No |
0 |
0 |
|
GEX + TCR/ GEX + BCR/ TCR + BCR |
16 |
Yes |
16 |
1 |
|
GEX + TCR + BCR |
16 |
Yes |
32 |
2 |
*Note that Single Cell 5' Library Construction Kits are only sold in whole number units. Fractions are used here for explanation only.
Products: Single Cell Immune Profiling