Question: Does the Visium for fresh-frozen Immunofluorescence Demonstrated Protocol differ from standard immunofluorescence protocols?
Answer: Yes, some components of the Visium with Immunofluorescence Demonstrated Protocol deviate from a standard immunofluorescence protocols such as the wash buffer used, antibody incubation time, and fixative.
Customers should perform the immunofluorescence staining as outlined in the demonstrated protocol. The immunofluorescence protocol has been optimized for optimal RNA quality to perform spatial gene expression analysis.
Products: Visium for fresh-frozen