Question: Is my antibody compatible with the Visium for fresh-frozen Immunofluorescence (IF) Staining Demonstrated Protocol?
Answer: Our Immunofluorescence (IF) Staining Demonstrated Protocol has been optimized for maintaining RNA quality to perform Visium Spatial Gene Expression analysis. To determine if your antibody(s) of interest is compatible with our recommended methanol-fixation and staining conditions, we advise that customers first test compatibility by following the demonstrated protocol to fix and stain their tissue on a plain glass slide prior to proceeding to the Visium Tissue Optimization or Gene Expression workflows. Additional information can be found in the demonstrated protocol.
Products: Visium for fresh-frozen