Question: Are fully custom panels supported for use in the Spatial Targeted Gene Expression assay?
Answer: We do not support the use of stand-alone fully custom panels with Visium Spatial Gene Expression libraries. However, customers can use the 10x Genomics Custom Panel Designer to customize 10x Genomics pre-designed panels to meet the needs of their experiments by adding up to 200 genes as add-ons to any of the 10x Genomics pre-designed panels.
We have observed poor signal-to-noise ratios when using fully custom panels targeting <1,000 genes with Visium Spatial Gene Expression libraries. To maximize signal-to-noise ratios, we recommend using large panel sizes, which can be achieved by using 10x Genomics pre-designed panels (which each target >1,000 genes), or by adding-on additional genes to 10x Genomics pre-designed panels.
Product: Targeted Gene Expression